Demeclocycline

Research use only, not for human use.

Demeclocycline (Declostatin) is a tetracycline antibiotic produced by a strain of Streptomyces aureofaciens.Demeclocycline has antimicrobial activity and inhibits the action of vasopressin on the renal tubules, leading to diuresis.

Demeclocycline is an orally active tetracycline antibiotic. Demeclocycline impairs protein synthesis by binding to the 30S ribosomal subunit to inhibit binding of aminoacyl tRNA. Demeclocycline shows anti-bacterial activitise to a wide variety of bacterial infections.

Demeclocycline (0-100 μM; 24 h) treatment reduces AQP2 abundance in mpkCCD cells.Demeclocycline (10 μM; 24 h) treatment promotes the activity of monocytes and macrophages.Demeclocycline (1-10 μM; 72 h) treatment directly affects the growth of brain tumorinitiating cells.Western Blot Analysis Cell Line:MpkCCD cells Concentration:0-100 μM Incubation Time:24 hours Result:Decreased AQP2 abundance in mpkCCD cells, with significant effects at 50 μM.Cell Viability Assay Cell Line:mouse bone marrow derived macrophages and monocytes Concentration:10 μM Incubation Time:24 hours Result:Enhanced TNF-α production and modulated monocyte functions.Cell Viability Assay Cell Line:brain tumorinitiating cells Concentration:1, 5, and 10 μM Incubation Time:72 hours Result:Inhibited cells growth in two ways: using monocytes as an intermediary, and directly by affecting the proliferation and sphere-forming capacity of brain tumorinitiating cells.

Demeclocycline (Intraperitoneal injection; 40 mg/kg; once daily; 48 h) treatment results in a significant reduction of hyponatremia and a significant correction of the hypoosmolality, and is not nephrotoxic.Animal Model:Male Wistar rats induced with hyponatremia Dosage:40 mg/kg Administration:Intraperitoneal injection; 40 mg/kg; once daily; 48 hours Result:Increased urine volume, decreased urine osmolality, and caused a significantly increased fractional excretion of water.Animal Model:Male Wistar rats induced with hyponatremiaDosage:40 mg/kg Administration:Intraperitoneal injection; 40 mg/kg; once daily; 48 hours Result:Indicated the effect in the renal inner medulla for AQP2 and AC5/6 specifically, and not secondary toxicity effect.

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GPCR/G Protein

Antibacterial

Antibacterial

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127-33-3

464.85

C21H21ClN2O8

>98% (HPLC)

In Vitro:?H2O : 40 mg/mL (86.05 mM; Ultrasonic) DMSO : 25 mg/mL (53.78 mM; Ultrasonic )

[H][C@]12C[C@@]3([H])[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]3(O)C(O)=C1C(=O)c1c(O)ccc(Cl)c1[C@H]2O

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(-20℃)

With Ice Pack

≥ 2 years