(E)-3-(3,4-dichlorophenyl)-N-(2-(4-(methylsulfonyl)piperazin-1-yl)-2-oxoethyl)acrylamide

Research use only, not for human use.

(E)-3-(3,4-dichlorophenyl)-N-(2-(4-(methylsulfonyl)piperazin-1-yl)-2-oxoethyl)acrylamide is a chemical compound.

(E)-3-(3,4-dichlorophenyl)-N-(2-(4-(methylsulfonyl)piperazin-1-yl)-2-oxoethyl)acrylamide is a chemical compound.

SR18662 (0-10 μM; 24-72 hours) significantly reduces growth and proliferation of CRC cells as compared to treatment with vehicle control, ML264 (HY-19994). It shows improved efficacy in reducing viability of multiple CRC cell lines.SR18662 (10 μM; 24-72 hours) shows a significant increase in the number of apoptotic cells at both early and late states in DLD-1 and HCT116 cells.SR18662 (1 μM; 72 hours) reduces the expression of cyclins (cyclins E, A2, and B1) and components of MAPK (p-Erk) and WNT signaling pathways (p-GSK3 β) in cells.Cell Viability Assay Cell Line:CRC cells Concentration:0-10 μM Incubation Time:24 hours, 48 hours, 72 hours Result:Induced anti-tumor activity in colorectal cancer cell lines.Apoptosis Analysis Cell Line:DLD-1 and HCT116 cells Concentration:10 μMIncubation Time:24 hours, 48 hours, 72 hours Result:Increased apoptosis of colorectal cancer cell lines.Western Blot Analysis Cell Line:DLD-1 and HCT116 cells Concentration:1 μM Incubation Time:72 hours Result:Reduced levels of cyclins E, A2, and B1??inhibits activity of MAPK, WNT/β-catenin signaling pathways and decreases the levels of cyclins.

SR18662 (intraperitoneal injection; 5-10 mg/kg; daily or twice daily; 5 days injection, days break, and 5 days) significantly reduces the growth of tumors in a mouse xenograft model. Animal Model:Nude mice with DLD-1 cells Dosage:5 mg/kg; 10 mg/kg; 25 mg/kg Administration:Intraperitoneal injection; 5mg/kg daily, 5mg/kg twice a day,10 mg/kg daily, 10 mg/kg twice per day, 25mg/kg daily, and 25 mg/kg twice per day; 5 days of injections, 2 days break, and 5 days of injections Result:Caused a significant dose-dependent inhibition of xenograft growth in mice.

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Others

Other Targets

α2-adrenoceptor

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2505001-62-5

420.31

C16H19Cl2N3O4S

>98% (HPLC)

In Vitro:?DMSO : 125 mg/mL (297.40 mM)

CS(N(CC1)CCN1C(CNC(/C=C/c(cc1)cc(Cl)c1Cl)=O)=O)(=O)=O

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(-20℃)

With Ice Pack

≥ 2 years