Coppersensor 1

Research use only, not for human use.

Coppersensor 1 is a boron dipyrromethene-based fluorescent sensor for selective and sensitive detection of copper(I) ions (Cu + ) in biological samples. Coppersensor 1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton.

Coppersensor 1 is a boron dipyrromethene-based fluorescent sensor for selective and sensitive detection of copper(I) ions (Cu + ) in biological samples. Coppersensor 1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton.(In Vitro):Coppersensor 1 (1 mM) matches the absorption maximum of the apo and Cu + -bound probe with 543 nm excitation.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).A. Preparation of reagent stock solutions for imaging: 1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.2. Prepare a 10 mM stock solution of CuCl or CuCl2.B. CS1 labeling of live cells: 1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherentcells for imaging are grown to 50-80% confluency.3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer. 4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.(Higher concentrations of dye may result in high levels of background fluorescence)5. Incubate for 5-20 min in the dark at 25 or 37℃.C. Imaging the CS1-labeled cells:1. Imageing in standard confocal microscope with 543 nm excitation.(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton) .

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CS1

Others

Other Targets

unfolded protein response (UPR)

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874748-20-6

629.81

C30H50BF2N3S4

>98% (HPLC)

In Vitro:?DMSO : 25 mg/mL (39.69 mM)

[F-][B+3]1([F-])[N]=2C(C(=C(C2C)CC)C)=C(C3=C(C(=C([N-]31)C)CC)C)CN(CCSCCSCC)CCSCCSCC

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(-20℃)

With Ice Pack

≥ 2 years