5-Hydroxy-2'-deoxyuridine

Research use only, not for human use.

5-Hydroxy-2'-deoxyuridine is a major oxidation product of 2'-Deoxycytidine and can be incorporated into DNA in vitro.

5-Hydroxy-2'-deoxyuridine is a major oxidation product of 2'-Deoxycytidine and can be incorporated into DNA in vitro.(In Vitro):Translesion synthesis past 5-Hydroxy-2'-deoxyuridine in 18- and 45-member oligodeoxyribonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 5-Hydroxy-2'-deoxyuridine in the template is sequence context-dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 5-Hydroxy-2'-deoxyuridine. However, in a second sequence context, dC is the predominant base incorporated opposite 5-Hydroxy-2'-deoxyuridine. In that same sequence context, dC is also the predominant nucleotide incorporated opposite 5-Hydroxy-2'-deoxyuridine.

To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 2'-deoxy-5-hydroxyuridine (5-OHdU) in two different sequence contexts, were used. Translesion synthesis past 2'-deoxy-5-hydroxyuridine (5-OHdU) in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) in the template is sequence context dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). However in a second sequence context, dC is the predominant base incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). In that same sequence context, dC is also the predominant nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU).

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5-OHdU

Cell Cycle/DNA Damage

Nucleoside Antimetabolite/Analog

KCNQ1

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5168-36-5

244.2

C9H12N2O6

>98% (HPLC)

In Vitro:?DMSO : 33.33 mg/mL (136.49 mM)

O=C1N([C@@]2(O[C@H](CO)[C@@H](O)C2)[H])C=C(O)C(=O)N1

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(-20℃)

With Ice Pack

≥ 2 years