5-ETHYNYL-2'-DEOXYURIDINE

Research use only, not for human use.

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor de novo DNA synthesis through click chemistry.

5-Ethynyl-2'-deoxyuridine (EdU) is a nucleoside analog of thymidine that is used to monitor de novo DNA synthesis through click chemistry.1,2 Like 5-bromo-2’-deoxyuridine (BrdU; Item No. 15580), EdU is incorporated into DNA during active DNA synthesis. It can then be detected using a fluorescent azide probe and copper catalysis using 1,3-dipolar cycloaddition.Modified nucleosides, including EdU, can be cytotoxic when added to cells for prolonged periods.(In Vitro):5-Ethynyl-2'-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system.PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.(In Vivo):The number of 5-Ethynyl-2'-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two month old female mice.

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PROTACs

PROTAC

Alkyl-Chain

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61135-33-9

252.22

C??H??N?O?

>98% (HPLC)

DMSO : 50 mg/mL (198.24 mM; Need ultrasonic)；H2O : 25 mg/mL (99.12 mM; Need ultrasonic)

O=C1NC(C(C#C)=CN1[C@H]2C[C@@H]([C@H](O2)CO)O)=O

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(-20℃)

With Ice Pack

≥ 2 years