Avicularin

Research use only, not for human use.

Avicularin exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 macrophage cells.

Avicularin exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 macrophage cells. Avicularin inhibits the accumulation of the intracellular lipids by decreasing C/EBPα-activated GLUT4-mediated glucose uptake in adipocytes and potently inhibiting fatty acid synthase.

Cell Proliferation Assay Cell Line:Huh7 cells Concentration:25, 50, 100 μg/mL Incubation Time:12, 24, 36 and 48 h Result:Inhibited cell proliferation in a dose-dependent manner.Western Blot Analysis Cell Line:RAW 264.7 cells Concentration:10, 30, 100, 300 μM Incubation Time:1 h Result:Inhibited LPS-induced protein expression of iNOS and COX-2, release of pro-inflammatory cytokine IL-1β, degradation of cytosolic IκB, and phosphorylation of ERK.RT-PCR Cell Line:3T3-L1 cells Concentration:50 μM Incubation Time:6 days Result:Decreased PPARγ, C/EBPα, and aP2 mRNA levels approximately 28.7, 69.5, and 18.3%, respectively.

Animal Model:ACLT (anterior cruciate ligament transection)-induced rats Dosage:0.5, 1, 2 mg/kg Administration:Injected into the articular cavity of the right knee, twice a week for 4 weeks.Result:Alleviated the tibial subchondral osteolysis, reduces bone loss, and increases the bone mass of tibial subchondral bone.Attenuated ECM degradation, the loss of Aggrecan and Collagen II in ACLT-induced rats. Decreased the MMP3 and MMP13 protein level. Animal Model:Rats with amyloid Beta-induced Alzheimer's disease Dosage:25, 50, and 100 mg/kg Administration:Oral administration, for 21 days Result:Enhanced cognition activity, and reversed the effects of amyloid beta-induced inflammatory response and excessive oxidative stress.

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Others

Other Targets

ERK

Others-Field

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572-30-5

434.35

C20H18O11

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (230.23 mM)

C1=CC(=C(C=C1C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)OC4C(C(C(O4)CO)O)O)O)O

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(-20℃)

With Ice Pack

≥ 2 years