Amantadine hydrochloride

Research use only, not for human use.

Amantadine Hydrochloride is an antiviral and an antiparkinsonian drug.

Amantadine Hydrochloride is an antiviral and an antiparkinsonian drug.(In Vitro):Amantadine hydrochloride (0-500 μM, 26 h) inhibits SARS-CoV-2 replication, with IC50 concentrations between 83 and 119 μM.Amantadine hydrochloride (0-100 μg/mL, 24-72 h) markedly inhibits the proliferation of HepG2 and SMMC 7721 cells.Amantadine hydrochloride (0-75 μg/mL, 48 h) arrests the cell cycle at the G0/G1 phase and induces apoptosis.Amantadine hydrochloride (0-75 μg/mL, 48 h) reduces the levels of the cell cycle related genes and proteins (cyclin D1, cyclin E and CDK2), reduces Bcl-2 and increases the Bax protein and mRNA levels.(In Vivo):Amantadine hydrochloride (25 mg/kg, IP, once daily for 3 days) inhibits surgery induced neuroinflammation and learning and memory impairment.

Cell Viability Assay Cell Line:Vero E6 cells Concentration:500 μM, 100 μM, 20 μM, 4 μM, and 8 nM Incubation Time:26 h Result:Caused a concentration-dependent reduction (IC50=83 μM) of viral nucleic acids in the supernatant 26 h after infection at 10-500 μM. Caused a concentration-dependent reduction (IC50=119 μM) of viral nucleic acids in the cytosol 26 h after infection.Cell Proliferation Assay Cell Line:Human HCC cell lines (HepG2 and SMMC-7721) and normal hepatocellular cells (L02 cells) Concentration:0, 1, 2, 5, 10, 25, 50 and 100 μg/mLIncubation Time:24, 48 and 72 h Result:Inhibited cellular proliferation in a time- and dose-dependent manner in HepG2 and SMMC-7721 cells.Cell Cycle Analysis Cell Line:HepG2 and SMMC-7721 cells Concentration:0, 10, 25, 50 and 75 μg/mL Incubation Time:48 h Result:Significantly increased the population of HepG2 and SMMC-7721 cells in the G0/G1 phase in a dose-dependent manner, and significantly decreased the number of HepG2 cells in the S phase.Apoptosis Analysis Cell Line:HepG2 and SMMC-7721 cells Concentration:0, 10, 25, 50 and 75 μg/mL Incubation Time:48 h Result:Markedly increased the percentage of apoptotic HepG2 and SMMC-7721 cells (early- and late-stage apoptosis) in a dose-dependent manner.Western Blot Analysis Cell Line:HepG2 and SMMC-7721 cells Concentration:0, 10, 25, 50 and 75 μg/mL Incubation Time:48 h Result:Showed downregulation of cyclin D1, cyclin E and CDK2, and showed a decrease in Bcl-2 levels and an increase of Bax levels in HepG2 and SMMC-7721 cells. RT-PCR Cell Line:HepG2 and SMMC-7721 cells Concentration:0, 10, 25, 50 and 75 μg/mL Incubation Time:48 h Result:Revealed an increase in Bax and decrease in Bcl-2 genes.

Animal Model:Fischer 344 rats (Four-month old, male, 290-330 g, 15 rats each group) Dosage:25 mg/kg Administration:IP, once daily for 3 days (the first dose at 15 min before surgery)Result:Inhibited surgery induced neuroinflammation and learning and memory impairment, increased GDNF (glial cell line-derived neurotrophic factor) that was co-localized with glial fibrillary acidic protein (an astrocytic marker) in the hippocampus.

Adamantylamine | Aminoadamantane | NSC 83653

GPCR/G Protein

Dopamine Receptor

Dopamine

Infection

——

665-66-7

187.71

C10H18ClN

>98% (HPLC)

Ethanol: 38 mg/mL (202.45 mM); Water: 38 mg/mL (202.45 mM); DMSO: 38 mg/mL (202.45 mM)

Cl.NC12CC3CC(CC(C3)C1)C2

——

(-20℃)

With Ice Pack

≥ 2 years