CHIR-99021

Research use only, not for human use.

A potent, specific GSK-3 inhibitor with IC50 of 5 nM and 10 nM for GSK3β and GSK3α, respectively.

A potent, specific GSK-3 inhibitor with IC50 of 5 nM and 10 nM for GSK3β and GSK3α, respectively; mimics Wnt signaling in preadipocytes, stabilizes free cytosolic beta-catenin and inhibits adipogenesis by blocking induction of C/EBPα and PPARγ; potentiates insulin activation of glucose transport and utilization in vitro and in vivo; also widely used for stem cell differentiation and naive stem cell generation.(In Vitro):Laduviglusib (1-10 μM, 3 days) reduces the viability of the ES-D3 cells with an IC50 of 4.9 μM.Laduviglusib (5 μM, 48 h) activates the canonical Wnt-pathway in ES-D3 cells and ES-CCE cells.Laduviglusib (3 μM, 4 days) inhibits ES cell differentiation into neural cells.Laduviglusib (1 μM, 2 weeks) inhibits adipogenesis by blocking induction of C/EBPα and PPARγ in 3T3-L1 preadipocytes.Laduviglusib (2.5 μM, 24 h) protects Lgr5+ cells against radiation-induced apoptosis.(In Vivo):Laduviglusib (30 mg/kg, p.o ) rapidly lowers plasma glucose.Laduviglusib (2 mg/kg, i.p.) protects mice against radiation-induced lethal GI injury.

Laduviglusib (GMP) (20 μM, 21 days) transdifferentiates mouse fibroblasts to neurons (determined by increased number of TAUEGFP-/TUJ1-positive neuronal cells).Laduviglusib (GMP) (3-5?μM, 2 days) induces somatic cells differentiation of pluripotent stem (PS) cells (the detailed method refers to the reference).Laduviglusib (GMP) (12?μM, 5 days) converts human fibroblasts (HFF) into functional cardiomyocytes.Laduviglusib (GMP) (3 μM, 4 days, hESCs) activates the canonical WNT signaling pathway, confirmed by β-catenin translocation into the nucleus.Laduviglusib (GMP) (in MEFs expressing MyoD) enhances the marked increase in the number of proliferative cells led by RepSox (GMP) (HY-13012G) together with Forskolin (HY-15371).Laduviglusib (GMP) (5 μM, 1 day) can be used to generate islets (hCiPSC-islets) from human chemically induced pluripotent stem cells (hCiPSC).

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CT99021 | CHIR99021 | CHIR 99021 | CT-99021

PI3K/Akt/mTOR signaling

GSK-3

GSK-3α|GSK-3β

Cancer

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252917-06-9

465.3379

C22H18Cl2N8

>98% (HPLC)

DMSO: ≥ 5.1 mg/mL

CC1=CN=C(N1)C1=CN=C(NCCNC2=NC=C(C=C2)C#N)N=C1C1=C(Cl)C=C(Cl)C=C1

3-Pyridinecarbonitrile, 6-[[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-

(-20℃)

With Ice Pack

≥ 2 years