Handelin

Research use only, not for human use.

Handelin has anti-inflammatory activity by inhibiting NF-κB activation and pro-inflammatory cytokine productions.

Handelin has anti-inflammatory activity by inhibiting NF-κB activation and pro-inflammatory cytokine productions.

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling.Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin.RT-PCR Cell Line:RAW 264.7 cells Concentration:10 μM, 20 μM, 40 μM Incubation Time:5 hours Result:iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed. Western Blot Analysis Cell Line:RAW 264.7 cells Concentration:10 μM, 20 μM, 40 μM Incubation Time:Result:Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model . Animal Model:Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan Dosage:10 mg/kg, 20 mg/kg Administration:Oral administration; for 30 mintues Result:Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

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Others

Other Targets

inflammation|NF-κB

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62687-22-3

552.66

C32H40O8

>98% (HPLC)

In Vitro:?DMSO : 16.67 mg/mL (30.16 mM)

C[C@]([C@]([C@@](OC1=O)([H])[C@@](C1=C)([H])CC[C@]2(O)C)([H])[C@@]32C4)(C=C3)[C@]54[C@@]([C@H]6OC(C)=O)([H])[C@]([C@](C(C)=CC7)([H])[C@]7([H])[C@](C)(O)C6)([H])OC5=O

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(-20℃)

With Ice Pack

≥ 2 years