Enmein

Research use only, not for human use.

Enmein shows a significant inhibitory effect toward human tumor cell K562 with IC(50) values ranging from 3.2 μg/ml to 8.2 μg/ml.

Enmein shows a significant inhibitory effect toward human tumor cell K562 with IC(50) values ranging from 3.2 μg/ml to 8.2 μg/ml.(In Vitro):Four new ent-kaurane diterpenoids, sculponeatins F-I (1-4), together with six known compounds, sculponeatin E (5), epi-nodosin (6), epi-nodosinol (7), Enmein (8), and macrocalyxoformins A and B (9 and 10), were isolated from the leaves of Isodon sculponeata. Also obtained were ursolic acid, 2alpha,3beta-dihydroxy-urs-12-en-28-oic acid, 2alpha,3beta,19alpha-trihydroxy-urs-12-en-28-oic acid, beta-sitosterol, daucosterol, quercetin, pedalitin, rosmarinic acid, caffeic acid and ethyl caffeic acid. Their structures were determined by spectral methods (1D-, 2D-NMR and MS). Some diterpenoids were tested for their cytotoxicity to inhibit three kinds of human tumor cells K562, A549 and T24.

Enmein shows significant inhibitory effect toward K562 with an IC50 value ranging from 3.2 μg/ml to 8.2 μg/ml.Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml.Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%. Cell Viability Assay Cell Line:Splenic lymphocyte cells Concentration:3.13, 6.25, 12.5, 25 μg/ml Incubation Time:48 hours Result:Exhibited an IC50 value of 17 μg/ml in splenic lymphocytes.Cell Cycle Analysis Cell Line:Splenic lymphocyte cells Concentration:5.5 μg/ml, 11 μg/ml, 22 μg/ml Incubation Time:48 hours Result:Caused S stage cells and G2/M stage cells decreased.

Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression. Animal Model:Balb/c mice Dosage:6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg Administration:Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days Result:Had inhibitory effect on IL-2 release in blood.

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Microbiology/Virology

Antifection

Anti-infection

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3776-39-4

362.422

C20H26O6

>98% (HPLC)

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(-20℃)

With Ice Pack

≥ 2 years