Cyclo(L-Phe-trans-4-hydroxy-L-Pro)

Research use only, not for human use.

Cyclo(L-Phe-trans-4-hydroxy-L-Pro) is a natural product from Phellinus igniarius.

Cyclo(L-Phe-trans-4-hydroxy-L-Pro) is a natural product from Phellinus igniarius.(In Vitro):We previously identified Streptomyces griseus as an anti-cancer agent (Kim et al., 2014). In this study, we isolated compounds from S. griseus and evaluated their anticancer effect and toxicity in vitro and in vivo. METHODS AND RESULTS: Preparative centrifugal partition chromatography (CPC) was used to obtain three compounds, cyclo(L-[4-hydroxyprolinyl]- L -leucine], Cyclo(L-Phe-trans-4-hydroxy-L-Pro) and phenethyl acetate (PA). We chose PA, which had the highest anticancer activity, as a target compound for further experiments. PA induced the formation of apoptotic bodies, DNA fragmentation, DNA accumulation in G0/G1 phase, and reactive oxygen species (ROS) formation. Furthermore, PA treatment increased Bax/Bcl-xL expression, activated caspase-3, and cleaved poly-ADP-ribose polymerase (PARP) in HL-60 cells. Simultaneous evaluation in vitro and in vivo, revealed that PA exhibited no toxicity in Vero cells and zebrafish embryos. CONCLUSIONS: We revealed, for the first time, that PA generates ROS, and that this ROS accumulation induced the Bcl signaling pathway.

Cyclo(L-phenylalanyl-trans-4-hydroxy-L-proline); L-Phe-trans-4-hydroxy-L-Pro

Others

Other Targets

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118477-06-8

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C14H16N2O3

>98% (HPLC)

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O[C@@H]1C[C@@H]2N(C1)C(=O)[C@H](Cc1ccccc1)NC2=O

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(-20℃)

With Ice Pack

≥ 2 years