5-Ethynyluridine can be used to label newly synthesized RNA. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs.

A chemotherapeutic prodrug of 5-flourouracil (5-FU) used in the treatment of cancers; a thymidylate synthase inhibitor.

6-O-Methyl Guanosine inhibit colony-forming ability in a malignant xeroderma pigmentosum cell line.